Many studies on memory and learning utilize the immediate early gene c-Fos as an indicator of recent synaptic activity. By quantifying c-Fos positive neurons, we can compare activity across different populations in the brain, and analyze co-labelled neurons with other useful cell markers such as BrdU and Doublecortin. Our lab…
One trick on the confocal microscope is to use a larger pinhole so that a greater thickness of the section is captured in the image. Images acquired this way are comparable to a bunch of thin sections that are then merged into a “z-stack” except that some of the tissue…
And now for a journey outside (rostral, to be precise) of my comfort zone. These three pictures show new neurons in the mouse olfactory bulb at successively greater magnifications. Probably inspired by the science magazine I read as a kid that would show high mag photos of everyday objects (with corresponding…